The fashion retailer has announced that the branch in the Darwin Centre will close on Saturday, October 15.
A sign posted in the window reads: "Hey Shrewsbury, we're closing from Saturday 15th October.
"You can still visit us at your nearest store, Telford.
"Thank you for shopping with us. We'll see you soon."
Large parts of the store are already empty, while many of the items remaining are heavily discounted as River Island clears out its remaining stock.
However, the store isn't like to be empty for long as HMV will be taking over the unit.
The previous store – in the town’s Pride Hill Centre – closed in January last year ahead of the centre’s redevelopment.
Speaking last month, an HMV spokesperson said: “We’re excited to return to Shrewsbury with a new shop in The Darwin Centre, creating new jobs in the local area and offering even more of the best in entertainment across pop culture, music, film and TV. We’ll be announcing our opening date very soon.”
On Friday an HMV spokeswoman said the retailer is aiming to open its new store in late November, however no date has yet been confirmed.
Shopping centre manager Kevin Lockwood added: "The new store will be bigger and better than before and in a prominent location. I cannot wait for them to reopen their doors – I know our customers will be thrilled to see them back, and it’s perfect timing ahead of Christmas."
River Island has been contacted for comment.