The petition entitled Save The Shropshire Badgers has been signed by nearly 500 people and will be presented at a meeting of Shropshire Council's environment scrutiny committee next week.
Campaigners, who were active against culling earlier this year, are fighting against the controversial method coming to Shropshire should Defra legislation become more wide spread.
They are calling on vaccination to be used to prevent the spread of bovine TB in cattle, and have also said no culling should take place on council owned land. It has already happened in other parts of the UK, including Somerset, and saw angry clashes between farmers and campaigners.
It comes after Liz Truss, who earlier this week replaced north Shropshire MP Owen Paterson as Environment Secretary, said future culls would be carried out in Gloucestershire.
Shropshire Wildlife Trust has also spoken out in favour of vaccination, which some believe is more effective at saving cattle from the disease. Badger marches took place in Shrewsbury town centre earlier this year and billboards were also placed by Station Bridge.